Journal: Neuroendocrinology

Article Title: Single-Cell RNA Sequencing and Its Applications in Pituitary Research

doi: 10.1159/000540352

Figure Lengend Snippet: Summary illustrating single studies in pituitary research

Article Snippet: Showing IL-6 activates PSC s postdamage, with this effect decreasing in aging due to higher IL-6 levels [ ] , Mus musculus , 10x Genomics , 26,115.

Techniques: Control, Functional Assay, Binding Assay, Cell Differentiation, Activity Assay, Gene Expression, Inhibition

Journal: BMC Cancer

Article Title: High glucose promotes the progression of colorectal cancer by activating the BMP4 signaling and inhibited by glucagon-like peptide-1 receptor agonist

doi: 10.1186/s12885-023-11077-w

Figure Lengend Snippet: qRT-PCR primer sequences

Article Snippet: Noggin (200nM)(ab281817, Abcam, USA), an inhibitor of BMP4, and active recombinant human BMP4 (100nM)(ab238298, Abcam, USA) were added to cell culture for 48 h for inhibition or overexpression of BMP4 respectively.

Techniques: Sequencing

Journal: BMC Cancer

Article Title: High glucose promotes the progression of colorectal cancer by activating the BMP4 signaling and inhibited by glucagon-like peptide-1 receptor agonist

doi: 10.1186/s12885-023-11077-w

Figure Lengend Snippet: The expression of BMP4 was increased in CRC according to the data from the TCGA database ( A ) BMP4 expression was investigated in different types of cancer in the TCGA database ( B , C ) The expression of BMP4 was significantly increased in COAD( B ) and READ( C ) tumor samples(all P < 0.01) in the TCGA database ( D , E ) The expression of BMP4 was negatively associated with overall survival probability and disease free survival probability(all p < 0.05) BLCA, Bladder Urothelial Carcinoma; BRCA, Breast invasive carcinoma; CESC, Cervical squamous cell carcinoma and endocervical adenocarcinoma; CHOL, Cholangio carcinoma; COAD, Colon adenocarcinoma; ESCA, Esophageal carcinoma; GBM, Glioblastoma multiforme; HNSC, Head and Neck squamous cell carcinoma; KICH, Kidney Chromophobe; KIRC, Kidney renal clear cell carcinoma; KIRP, Kidney renal papillary cell carcinoma; LIHC, Liver hepatocellular carcinoma; LUAD, Lung adenocarcinoma; LUSC, Lung squamous cell carcinoma; PAAD, Pancreatic adenocarcinoma; PRAD, Prostate adenocarcinoma; PCPG, Pheochromocytoma and Paraganglioma; READ, Rectum adenocarcinoma; SARC, Sarcoma; SKCM, Skin Cutaneous Melanoma; THCA, Thyroid carcinoma; THYM, Thymoma; STAD, Stomach adenocarcinoma; UCEC, Uterine Corpus Endometrial Carcinoma

Article Snippet: Noggin (200nM)(ab281817, Abcam, USA), an inhibitor of BMP4, and active recombinant human BMP4 (100nM)(ab238298, Abcam, USA) were added to cell culture for 48 h for inhibition or overexpression of BMP4 respectively.

Techniques: Expressing

Journal: BMC Cancer

Article Title: High glucose promotes the progression of colorectal cancer by activating the BMP4 signaling and inhibited by glucagon-like peptide-1 receptor agonist

doi: 10.1186/s12885-023-11077-w

Figure Lengend Snippet: BMP4 expression was significantly higher in CRC patients with DM than in CRC patients without DM. ( A ) The plasma level of BMP4 in CRC patients with DM (CA + DM group) was significantly higher than patients without DM (CA group), *compared to the CA group, P < 0.05 ( B , C ) The upregulation of the protein and RNA expression of BMP4 was confirmed by western blotting and qRT-PCR in CRC specimens from patients with or without DM, *compared to the CA group, P < 0.05 ( D ) Immunohistochemical localization of BMP4 in tumor samples of CRC patients with or without DM (scale bar = 50 μm)

Article Snippet: Noggin (200nM)(ab281817, Abcam, USA), an inhibitor of BMP4, and active recombinant human BMP4 (100nM)(ab238298, Abcam, USA) were added to cell culture for 48 h for inhibition or overexpression of BMP4 respectively.

Techniques: Expressing, Clinical Proteomics, RNA Expression, Western Blot, Quantitative RT-PCR, Immunohistochemical staining

Journal: BMC Cancer

Article Title: High glucose promotes the progression of colorectal cancer by activating the BMP4 signaling and inhibited by glucagon-like peptide-1 receptor agonist

doi: 10.1186/s12885-023-11077-w

Figure Lengend Snippet: High glucose promoted the proliferation and metastasis of CRC cells ( A ) Glucose consumption was measured in different types of CRC cell lines by glucose assay kit, *P < 0.05, ***P < 0.001 ( B ) The result of glucose consumption was rectified by cell viability measured by CCK-8 kit (OD 450), *P < 0.05, ***P < 0.001 ( C ) Protein of BMP4 expression examined by western blotting in different types of CRC cell lines ( D , E ) Treated with high glucose(50mM) 48 h could upregulate the expression of BMP4 measured by western blotting( D ) and qRT-PCR( E ), *P < 0.05 ( F ) Cell viability of SW1116 and MC38 cultivated by high glucose(50mM) and Noggin(200nM) for 48 h assessed by CCK-8kit, *P < 0.05, ***P < 0.001 ( G ) Transwell assays were performed to examine the potential migration of SW1116 and MC38 cells treated with or without high glucose(50mM) and Noggin(200nM).

Article Snippet: Noggin (200nM)(ab281817, Abcam, USA), an inhibitor of BMP4, and active recombinant human BMP4 (100nM)(ab238298, Abcam, USA) were added to cell culture for 48 h for inhibition or overexpression of BMP4 respectively.

Techniques: Glucose Assay, CCK-8 Assay, Expressing, Western Blot, Quantitative RT-PCR, Migration

Journal: BMC Cancer

Article Title: High glucose promotes the progression of colorectal cancer by activating the BMP4 signaling and inhibited by glucagon-like peptide-1 receptor agonist

doi: 10.1186/s12885-023-11077-w

Figure Lengend Snippet: High glucose promoted the EMT of SW1116 and MC38 cells through the BMP4-Smad pathway ( A ) Western blotting analysis of the expression of EMT markers in SW1116 and MC38 cells cultivated with or without high glucose(50mM) and Noggin(200nM). ( B ) High glucose activated the SMAD1/5/8 and this effect could be blocked by Noggin proved by western blot analysis ( C ) Western blotting analysis of the efficiency of sh-BMP4 and vector transfection in SW1116 cells ( D ) Western blotting analysis of the expression of BMP4, EMT markers and pSMAD1/5/8 in SW1116 transfected with sh-BMP4-2 treated with or without high glucose(50mM) for 24 h

Article Snippet: Noggin (200nM)(ab281817, Abcam, USA), an inhibitor of BMP4, and active recombinant human BMP4 (100nM)(ab238298, Abcam, USA) were added to cell culture for 48 h for inhibition or overexpression of BMP4 respectively.

Techniques: Western Blot, Expressing, Plasmid Preparation, Transfection

Journal: BMC Cancer

Article Title: High glucose promotes the progression of colorectal cancer by activating the BMP4 signaling and inhibited by glucagon-like peptide-1 receptor agonist

doi: 10.1186/s12885-023-11077-w

Figure Lengend Snippet: GLP-1RA diminished the proliferation of CRC. ( A ) The expression of GLP-1R was downregulated by high glucose presented by western blot ( B ) Western blotting analysis of the expression of BMP4 in SW1116 and MC38 treated with GLP-1RA, liraglutide, with different concentrations (0, 10, 20, 50, 100nM) for 48 h ( C ) The dead cells of SW1116 and MC38 intervened with GLP-1RA for 48 h were detected by Calcein‑AM/PI double staining (magnification, x100) ( D , E ) Cell viability of SW1116 and MC38 cells treated with GLP-1RA for 48 h were measured by CCK-8 kit, *P < 0.05, ***P < 0.001

Article Snippet: Noggin (200nM)(ab281817, Abcam, USA), an inhibitor of BMP4, and active recombinant human BMP4 (100nM)(ab238298, Abcam, USA) were added to cell culture for 48 h for inhibition or overexpression of BMP4 respectively.

Techniques: Expressing, Western Blot, Double Staining, CCK-8 Assay

Journal: BMC Cancer

Article Title: High glucose promotes the progression of colorectal cancer by activating the BMP4 signaling and inhibited by glucagon-like peptide-1 receptor agonist

doi: 10.1186/s12885-023-11077-w

Figure Lengend Snippet: GLP-1RA promoted the apoptosis of CRC cells mediated by the dimmish of BMP4. ( A ) GLP-1RA promotes the apoptosis of SW1116 examined by flow cytometric analysis ( B ) Cell viability of SW1116 and MC38 cell lines cultivated by GLP-1RA and recombinant BMP4 for 48 h assessed by CCK-8kit ( C ) Western blot analysis of the expression of BCL-2, Caspase-3, and cleave-Caspase-3 in SW1116 and MC38 cell lines treated with GLP-1RA and recombinant BMP4.

Article Snippet: Noggin (200nM)(ab281817, Abcam, USA), an inhibitor of BMP4, and active recombinant human BMP4 (100nM)(ab238298, Abcam, USA) were added to cell culture for 48 h for inhibition or overexpression of BMP4 respectively.

Techniques: Recombinant, Western Blot, Expressing

Journal: BMC Cancer

Article Title: High glucose promotes the progression of colorectal cancer by activating the BMP4 signaling and inhibited by glucagon-like peptide-1 receptor agonist

doi: 10.1186/s12885-023-11077-w

Figure Lengend Snippet: High glucose promoted the metastasis of CRC validated in vivo studies ( A ) Protocol for high-fat diet (HFD) and STZ-induced diabetes mouse models and liver metastasis model ( B ) Representative image of the liver of mice with diabetes (DM + CA group) and without diabetes (CA group) ( C ) BMP4 and E-cadherin, N-cadherin, Vimentin protein levels in formalin-fixed, paraffin-embedded tumors as detected by IHC.

Article Snippet: Noggin (200nM)(ab281817, Abcam, USA), an inhibitor of BMP4, and active recombinant human BMP4 (100nM)(ab238298, Abcam, USA) were added to cell culture for 48 h for inhibition or overexpression of BMP4 respectively.

Techniques: In Vivo, Formalin-fixed Paraffin-Embedded

Journal: BMC Gastroenterology

Article Title: Triptolide attenuates irritable bowel syndrome via inhibiting ODC1

doi: 10.1186/s12876-023-02847-8

Figure Lengend Snippet: The effects of triptolide administration on inflammatory cytokines expression in rats with CAS-inudced IBS. ELISA was conducted to measure the expression of IL-1, IL-6, and TNF-α in the ileum (A) and colon (B) in each group. Results are expressed as mean ± SD (n = 6). ** P < 0.01 vs. Control group, ## P < 0.01 vs. IBS group

Article Snippet: The IL-1, IL-6, and TNF-α Active ELISA (Active Motif, USA) kit was used to measure the binding activity of free IL-1, IL-6, and TNF-α in ileum and colon.

Techniques: Expressing, Enzyme-linked Immunosorbent Assay, Control